Figure 2.
Expression and subcellular localisation of mini-MUC4 in pancreatic adenocarcinoma cell lines. (A) A total of 10 μg protein from cell extracts were resolved by electrophoresis on a 2% SDS-agarose gel containing Tris–glycine (pH 8.8) buffer, transferred to PVDF membrane and incubated with anti-MUC4 monoclonal antibody. The membrane was then probed with HRPO-labelled goat anti-mouse Ig. Immunoblot of actin, obtained from 10% SDS-polyacrylamide gel, was used as an internal control. (B) Confocal photomicrograph demonstrating expression pattern of MUC4 in methanol-fixed human pancreatic tumor cells. The cells were grown at low density on coverslips for 24 h, and the acetone/methanol (1 : 1)-fixed cells were labelled using a FITC-conjugated anti-MUC4 monoclonal antibody and counterstained by propidium iodide. Magnification, × 630.