Figure 1.
Construction of the mini-MUC4. (A) Schematic representation of cloning strategy for the generation of the mini-MUC4 construct. Fragments amplified by PCR were subcloned in PCR2.1 vector, sequenced, digested with specific restriction sites, and subcloned in the pBluescript vector. Finally, the 5′ region, JER64 fragment, and the 3′ fragments were subcloned in the pSecTag C vector. Each junction was sequenced to confirm the reading frame. (B) A schematic representation of mini-MUC4 gene. The sequence of the mini-MUC4 (320 kDa) is comparable with that of wild-type MUC4 (930 kDa), but with a repetitive domain representing only 10% the normal size.
