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Inactivation of p53 in human LS180 cells induces expression of MDR1. (A) Northern blot analysis was performed on 25 μg of total RNA from each cell line. The membrane was probed sequentially with a cDNA fragment for human MDR1 (49) followed by a cDNA for glyceraldehyde 3-phosphate dehydrogenase (GAPDH). (B) Immunoblot analysis of PGP in total cell lysates from Neo-1, Neo-3, TDN-2, TDN-4, and TDN-5 was performed as described in Materials and Methods.
