Figure 3.
Inactivation of wild-type p53 is associated with elevated levels of MDR1 gene expression. (A) Immunoblot analysis of Pgp and thymidylate synthase in total cell lysates from Neo-6, Neo-17, TDN-7, TDN-8, and TDN-14 was performed as described in Materials and Methods. (B) Total RNA (2 or 10 μg) isolated from Neo-6, Neo-17, TDN-7, TDN-8, and TDN-14 was slot-blotted and hybridized with a mdr1a-specific oligonucleotide as described in Materials and Methods. The blot was stripped and reprobed with a cDNA for EI24.