Figure 4.
Effect of the various BPI and/or LBP species on the triggering of lucigenin- (A and C) or luminol (B and D)-enhanced leukocyte chemiluminescence by purified LPS (A and B) or E. coli K1/r (C and D). Bacteria or LPS were preincubated plus or minus the indicated protein for 30 min before addition to leukocytes plus lucigenin or luminol and measurement of chemiluminescence as described in Materials and Methods. The results shown represent the mean of four determinations using data obtained during incubation of 10 ng/ml LPS or 106/ml E. coli with leukocytes.