Table 2.
Properties of PKC isoforms recovered from the H2O2-treated cells
| PKC isoform | H2O2 treatment, min | Activators | PKC activity
|
|
|---|---|---|---|---|
| PSL | % | |||
| δ | 0 | — | 9,100 | 100 |
| δ | 0 | PS, DG | 37,400 | 441 |
| δ | 10 | — | 41,900 | 460 |
| δ | 10 | PS, DG | 40,600 | 446 |
| α | 0 | — | 20,500 | 100 |
| α | 0 | PS, DG, Ca2+ | 133,000 | 650 |
| α | 10 | — | 86,100 | 420 |
| α | 10 | PS, DG, Ca2+ | 80,000 | 390 |
Phosphorylation of H1 histone by the PKC isoforms described in Fig. 3 in the presence or absence of phosphatidylserine (PS), diacylglycerol (DG), and Ca2+ was quantitated by measuring PSL using a Bio-Imaging Analyzer. The activity of each PKC isoform recovered from the cells without H2O2 treatment in the absence of these activators was used as 100%.