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PBMCs from HIV env DNA plus o-gp160-immunized rhesus monkeys were negative for SIV gag/pol DNA by PCR analysis. DNA was isolated from PBMCs of the experimental vaccinated monkeys (0092 and 0003) and two of the control naive/vector DNA plus ovalbumin-vaccinated monkeys (0059 and 0053) at the times noted and subjected to PCR amplification using oligomers specific for either SIV gag/pol or, as a control, the cellular CD4 gene.
