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. 1999 Oct 26;96(22):12743–12748. doi: 10.1073/pnas.96.22.12743

Figure 2.

Figure 2

(A) A large var transcript is detected in total RNA of FCR3-CSA trophozoites. Lanes: 1, FCR3.varCSA DBL-1 sequence probe; 2, Hsp70-specific probe hybridizing to the P. falciparum heat shock gene transcript of ≈3 kilobases. (B) Identification of a high molecular weight 125I-labeled and trypsin-sensitive erythrocyte surface molecule. Shown is the separation of SDS extracts of surface iodinated FCR3-CSA (first lane) and FCR3-CD36 (third lane) trophozoites. The labeled high molecular mass proteins of ≈400 and 250 kDa are sensitive to trypsinization (second lane and fourth lane). (C) CSA carrying human thrombomodulin (hTM)-coated Dynabeads affinity-purifies a high molecular weight molecule in SDS extracts from FCR3-CSA but not from FCR3-CD36 trophozoites. The purified molecule is sensitive to trypsin treatment. 125I-PfEMP-1 molecules are indicated by arrows.