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Expression of human FVIII in mouse plasma. C57BL/6 mice were injected via the portal vein with either 3 × 1011 particles of rAAV-hFVIII-HC, 3 × 1011 particles of rAAV-hFVIII-LC, 3 × 1011 particles of both rAAV-hFVIII-HC and rAAV-hFVIII-LC, or 3 × 1011 particles of rAAV-hFIX. Blood samples were collected via the retroorbital, and plasma was analyzed by ELISA for the level of hFVIII light chain antigen.
