Figure 1.
Cloning of a Rb-interacting protein, Trip230. (A) The corresponding mRNA of Trip230 was detected by using poly(A)+ RNA isolated form human cell lines SW620 (lane 1) and T47D (lane 2) and Northern blot analysis. The 0.9-kb cDNA fragment originally isolated from yeast two-hybrid screen was used as a probe. Two major transcripts of 6.4 kb and 4 kb were identified as indicated. (B) The same 0.9-kb cDNA probe was used to first localize the gene encoding Trip230 to chromosome 14 by Southern blot analysis. Human metaphase spreads then were analyzed by in situ hybridization using the same 0.9-kb cDNA fragment as a probe to map the gene to chromosome 14q31. (C) Amino acid sequence of Trip230 as deduced from the cloned cDNA sequence.