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. 2008 Apr 11;28(4):559–570. doi: 10.1016/j.immuni.2008.02.019

Figure 3.

Figure 3

Absence of TGF-β Signaling in T Cells Increases Intestinal Inflammation in Il23a−/−Rag1−/− Mice

(A) Colitis score of Il23a−/−Rag1−/− mice transferred with wild-type (WT) or dnTGFβRII (DN) naive T cells. Each point represents an individual mouse. Below: Representative microphotographs of colonic sections from Il23a−/−Rag1−/− mice transferred with WT (score 2) or DN CD4+CD45RBhi T cells (score 5). The scale bars represent 200 μm.

(B) IL-17 and IFN-γ production in Il23a−/−Rag1−/− mice transferred with wild-type (WT) or dnTGFβRII (DN) naive T cells. The figure shows representative FACS plots of IL-17 and IFN-γ production by MLN lymphocytes gated on CD4+ cells and the concentrations of IL-17 and IFN-γ (mean + SEM) in the colon of transferred Il23a−/−Rag1−/− mice.

(C) Foxp3 expression in CD4+ cells after transfer. Left: Representative FACS plots showing Foxp3 frequency in MLN from transferred Il23a−/−Rag1−/− mice. Plots are gated on CD4+ TCRβ+ cells. Right: Percentage of Foxp3+ among MLN CD4+ T cells of transferred Il23a−/−Rag1−/−. Each point represents an individual mouse. Data are pooled from three independent experiments. ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001.