TABLE 3.
Comparison of semiquantitative RT-PCR and quantitative and nonamplified mRNA detection on microarrays as indicated by the induction (n-fold) of gene expression
| Gene | Fold inductiona 48 h postinfection as determined by:
|
||
|---|---|---|---|
| SYBR Green analysis | RT-PCR analysis | Affymetrix analysis | |
| h-IAP1 | 8.1 | 7.79 | 11 |
| 2′-5′ OAS | 72.8 | >10 | 67 |
| 2′-5′ OASL | >100 | >10 | 16 |
| RGS2 | 2.8 | 3.9 | <4 |
| Gal-9 | 4.59 | 4.7 | 6.5 |
| MxA | >100 | >11 | 48.5 |
| ESDN | ND | 0.45 | <4 |
| TNF-α | 3.76 | 3.82 | <4 |
| IL-1β | 40.77 | 10.23 | 9.4 |
| TLR3 | ND | ND | 14 |
| PLSCR1 | ND | >10 | 7.9 |
a
ND, not determined.