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. 2008 Apr 14;205(4):869–882. doi: 10.1084/jem.20071087

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Copyright © 2008, The Rockefeller University Press

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Figure 7. — The alum response in mice depends on uric acid and MyD88 signaling. (A) Mice were injected with saline, OVA, or OVA-alum, and after 2 h, uric acid levels were determined in the peritoneal lavage. Data are shown as the mean ± the SEM. **, P < 0.01. n = 5–6 mice per group. (B) Mice were injected with uricase 1 d and 5 min before OVA-AF647 or OVA-AF647-alum, and 24 h later, the DLNs (MLN) were taken. The number of OVA-AF647⁺ inflammatory monocytes (defined as CD11b⁺Ly6C^highLy6G⁻F4/80^int) are shown. Data are shown as the mean ± the SEM. *, P < 0.05. n = 4–5 mice per group. (C) At day 0, mice received a cohort of CFSE-labeled DO11.10 T cells i.v. and uricase i.p. At day 1, mice received another injection with uricase, and 5 min thereafter OVA or OVA-alum i.p. 4 d after the last injection, proliferation of Tg T cells were determined in the draining MLN. Percentages in the plots are the percentage of Tg cells from total CD4⁺ T cells. An example is shown from four mice, and the experiment was repeated twice. (D) Quantification of the number of Tg cells from 4 and 7 d after OVA or OVA-alum plotted in C. Data are shown as mean ± SEM. *, P < 0.05; **, P < 0.01. n = 4 mice per group. (E) MyD88^−/− and WT mice were injected with OVA-AF647 or OVA-AF647-alum, and 24 h later, the DLN (MLN) were examined. The number of OVA-AF647⁺ inflammatory monocytes (defined as CD11b⁺Ly6C^highLy6G⁻F4/80^int) are shown. Data are shown as mean ± SEM. *, P < 0.05. n = 4 mice per group.