FIG. 1.
Correlation between Nef and GFP expression. (A) HeLa-CIITA cells and Jurkat cells were transfected with a bicistronic vector coexpressing GFP and NL4-3 Nef (upper panel) or GFP alone (lower panel). As described in Materials and Methods, cells were permeabilized, and Nef and GFP expression was detected simultaneously by two-color flow cytometric analysis. Ranges for green fluorescence on cells defined as expressing no (N), low (L), medium (M), or high (H) levels of GFP are indicated. (B) Quantitation of Nef expression in the transiently transfected cells shown in panel A. The ordinate gives the mean channel numbers of red Nef fluorescence for the four different ranges of GFP expression.