FIG. 5.
Comparison of the MHV-68 transcription program at the peak of gene expression in vivo and in vitro. Total RNA was harvested from BHK-21 cells at 32 hpi or from the lungs of BALB/c mice at 5 dpi, and labeled cDNA probe was generated for hybridization to MHV-68 membrane arrays. The GAPDH-normalized phosphorimager units for each array element were divided by the total counts across the entire corresponding array. (A) These values and their corresponding MHV-68 ORFs are ordered in the bar graph based on decreasing gene expression levels in the lung (▪) and show the corresponding value in BHK-21 (░⃞). For ease of viewing, the values are illustrated in two graphs. (B) The Spearman rank correlation coefficient was used to assess the relationship between the magnitude of genes expressed in cell culture and in the lung of infected mice at the peak of viral gene expression.