Figure 6. MCH-mediated depression of frequency and amplitude of mEPSCs.
A, raw traces presented here were recorded in the presence of TTX (1 μm) and BIC (30 μm) under voltage clamp (-60 mV). B, normalised frequency of mEPSCs from two neurons was plotted to show the time course of MCH actions. C, normalised frequency of mEPSCs from all eight neurons was plotted in this bar graph. D, a representative cumulative distribution from five of eight neurons showing that the cumulative probability of detected events was changed during the application of MCH. E, a representative cumulative distribution from three of eight neurons showing that the cumulative probability of detected events was not changed during the application of MCH, although the frequency was reduced by 15 %. F, mean normalised median of mEPSC amplitudes before, during and after MCH treatment was plotted.