Figure 2. E2 inhibits forskolin-induced Cl− secretion.
A, representative effect of forskolin (Fsk, 20 μm) on ISC of female rat distal colonic epithelium in the absence (continuous line) and presence (dotted line) of E2 (100 nm). Both tissues remain sensitive to bumetanide (Bumet, 100 μm). B, mean +s.e.m. (n = 6%) change in ISC caused by forskolin in the presence of varying concentrations of E2. *Significant difference at the level of P≤ 0.05 compared to control (0 nm E2) values; **significant difference at the level of P≤ 0.01 compared to control (0 nm E2) values.