Figure 3. NEM does not block the facilitatory action of ATP on mIPSC frequency.
Aa, mIPSCs recorded in the presence of NEM (left) and in the presence of NEM and ATP (right). Ab, normalized cumulative amplitude distributions (left) and cumulative frequency distributions (right) of mIPSCs in external solution with NEM, with or without ATP. Insets show the standard histograms of amplitude and inter-event intervals for control (thin line) and ATP (thick line) from the same neuron. B, summary of mean data illustrating ATP and adenosine actions on mIPSCs in the presence or absence of NEM. The relative iGly (left) shows the amplitude ratio of mean glycinergic mIPSCs before and during application of ATP or adenosine. The relative frequency (right) shows the frequency ratio of mean glycinergic mIPSCs before and during application of ATP or adenosine (**P < 0.001, n = 4). In the presence of NEM, ATP still potentiated mIPSC frequency without altering the current amplitude distribution. On the other hand, NEM completely blocked the inhibitory effect of adenosine on mIPSC frequency.