Figure 3.
Heterochromatin protein 1 factors colocalize with RNA polymerase II on HIV1 chromatin. After 2 h of PMA treatment of J-Lat A1 cells, chromatin immunoprecipitation experiments were performed with antibodies specific for HP1β, HP1γ or RNAPII amino terminus. Enrichments after immunoprecipitation from (A) unstimulated or (B) PMA-treated cells were measured using quantitative PCR. (C) Schematic representation of PCR amplicons on the HIV1 construct. HP1, heterochromatin protein 1; LTR, long terminal repeat; PMA, phorbol-myristate acetate; RNAP, RNA polymerase II.