FIG. 4.
B6.PD-1 KO mice develop more dramatic spleen cell phenotypic changes than WT B6 mice after LP-BM5 infection. B6.PD-1 KO and WT B6 mice were infected with 2 × 104 PFU LP-BM5 virus and sacrificed at 3.5 or 8 wpi. Spleen cells labeled in two-color analyses with MAb to cell surface markers CD23 and CD19 (A) or CD16/CD32 and CD11b (B) were analyzed on a FACScalibur flow cytometer. The percentages of total CD19+ cells that are also CD23+ are shown in the upper right quadrants of panel A, and the percentages of total CD16/CD32+ cells that are also CD11b+ are shown in the upper right quadrants of panel B. A log fluorescence scale was used. This pattern of results was also observed in two other experiments.