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. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: Immunity. 2008 Jan;28(1):100–111. doi: 10.1016/j.immuni.2007.11.021

Figure 6. IL-2 and IL-15 are the predominant common γ-chain cytokines which induce Foxp3.

Figure 6

(A) Analysis of common γ-chain cytokines for their ability to induce Foxp3. Sorted CD25hi Foxp3− CD4SP cells were assayed with the indicated murine cytokines (100 ng/ml) and IL-7 (5 ng/ml) as described in Figure 4A. Titration of cytokines down to 1 ng/ml did not enhance the percentage of Foxp3+ cells observed (data not shown). (B) IL-2 is more potent than IL-15 for the induction of Foxp3. Purified CD25hi Foxp3− CD4SP thymocytes were cultured with IL-7 (5 ng/ml) and indicated concentrations of murine IL-2 or IL-15 and analyzed for Foxp3 expression by flow cytometry at 24 hours. Data is representative of two independent experiments. The difference in potency between IL-2 and IL-15 was calculated using the concentration at half maximal Foxp3 induction.