Figure 3.
PIKfyve is the cellular target of YM201636. (A) Phase-contrast images of NIH3T3 cells transfected with siRNA to PIKfyve; the insets show magnified views. A 25% portion of the cells showed this vesiculation phenotype, which is consistent with the transfection efficiency; no such behaviour was observed with non-PIKfyve-directed siRNA. The siRNA used is shown above the images. (B) pEGFP or GFP-Fab1 fusion proteins were expressed in NIH3T3 cells and the cells were treated for 2 h with 800 nM YM201636. The data are from three independent transfections. EGFP, enhanced GFP; GFP, green fluorescent protein; PIKfyve, mammalian type III phosphatidylinositol phosphate kinase; siRNA, short interfering RNA.