FIG. 1.
Identification of LNA-interacting proteins by yeast two-hybrid screening. Full-length LNA (KSHV ORF73) was cloned into pAS2-1 vector (A). Detection of LNA GAL4 DBD fusion protein in Y190 yeast cells transformed with pAS2-1-LNA by Western blot assay (B). The arrowhead indicates the LNA band (lane 3). Y190 alone (lane1) and Y190 transformed with a recombinant pAS2-1 plasmid containing vIRF (KSHV ORF-K9) gene (lane 2) were used as negative controls. (C) The prey DNA was extracted from the positive clones after initial screening and transformed with either LNA bait construct or negative control vIRF construct to confirm the interaction. The novel clone was shown as a representative result.