Figure 2.
Identification of a novel acetylation site in MEF2C. (A) MALDI spectra (range 1265–1290 kDa) of FLAG-MEF2C tryptic digests obtained from transfected C2C7 myoblasts (Mb, upper panel) and myotubes (Mt, lower panel). The monoisotopic peaks at 1275.7 and 1273.7 kDa are indicated. (B) A table is shown indicating the mass of the monoisotopic ions (MH+), the residues range (from aa to aa), the theoretical molecular weight (Th MH+), the sequence of unmodified peptides and compatible modifications. The residues amenable to the indicated modifications are underlined. (C) Sequence comparison between the MADS box of MEF2 proteins and of other members of the MADS box family. The lysine 4 residue is highlighted.