Table 3.
Release Calculation Algorithm Applied to Different Images from Permeabilized Fibers
| Sparkamplitude | Peakfluxdensity | Release | |||||
|---|---|---|---|---|---|---|---|
| Image | dye T | Current | FWHM | FDHM | Dyeterm | ||
| μM | mM/s | pA | μm | ms | pA | ||
| 812a2 | 1.9 | 377 | 154 | 14.7 | 0.55 | 8.5 | 10.8 |
| 812b7 | 1.5 | 256 | 81 | 16.6 | 0.90 | 6.1 | 3.19 |
| 812c12* | 2.2 | 356 | 92 | 15.8 | 1.10 | 7.3 | 14.8 |
| 812d13 | 2.1 | 328 | 114 | 14.6 | 0.93 | 4.6 | 11.0 |
| 813a2 | 1.7 | 339 | 91 | 13.9 | 0.96 | 6.9 | 8.40 |
| 813a6 | 2.8 | 325 | 88 | 20.7 | 1.18 | 6.7 | 14.4 |
| 813b1 | 2.7 | 235 | 154 | 18.1 | 0.81 | 6.7 | 7.84 |
| 817a76 | 1.8 | 184 | 83 | 11.9 | 1.00 | 8.6 | 5.74 |
| 817a76 | 2.2 | 184 | 112 | 17.0 | 0.83 | 8.4 | 2.84 |
| 817b10 | 1.9 | 170 | 185 | 7.4 | 0.69 | 9.8 | 4.74 |
| 817b12 | 2.2 | 177 | 152 | 14.2 | 0.54 | 8.8 | 8.96 |
| 819c22 | 2.9 | 169 | 176 | 15.3 | 0.76 | 8.8 | 2.69 |
| 819c22 | 2.7 | 166 | 146 | 14.1 | 0.86 | 8.1 | 4.04 |
| 819c28 | 2.5 | 161 | 145 | 12.3 | 0.77 | 7.6 | 6.49 |
| 820b20 | 1.6 | 230 | 96 | 15.3 | 1.11 | 6.9 | 3.49 |
| 824a45 | 1.3 | 211 | 112 | 5.0 | 0.57 | 6.4 | 3.55 |
| Average | 2.14 | 260 | 119 | 14.4 | 0.89 | 7.5 | 8.28 |
| SEM | 0.10 | 19.0 | 7.8 | 0.81 | 0.05 | 0.27 | 1.07 |
The first number and letter in column 1 identify the experiment, the last number in column 1 identifies the image. Repeated numbers identify sparks from the same image. *Average of four sparks, represented in Fig. 8 and Fig. 9. Column 2 lists amplitude of the event, calculated as in Table . dye T was calculated using . Current, FWHM, and FDHM were calculated on the release flux waveform, derived after smoothing and without deblurring. The last column lists the dye-related estimate of release current illustrated in Fig. 9.