Figure 3.

Release analysis of a simulated spark with noise. (A) Fluorescence spark of Fig. 2 (0.2-μm source radius, 20 pA/5 ms Ca2+ current, with removal parameters listed in Table , blurred as described) plus normally distributed noise of
. (B and C) Representations of the release flux derived by the algorithm. (D) Temporal and (E) spatial profiles of the calculated release flux density (thick line) and the flux used to generate the spark (thin line). (F) Derived release current (thick line), and release current used in the simulation (thin line). The application of the algorithm included partial deblurring, with
(see text for details).