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. 1998 Oct 1;112(4):409–422. doi: 10.1085/jgp.112.4.409

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Open times of flickering pores can be switched during flickering. (top) R18 or DiI were preferentially located in the trans monolayer (−20 mV). After the first flickering pore closed, voltage polarity was changed to 20 mV to move probe to the cis monolayer and open times of pores that followed were obtained. For DiI (right), the first new pore that formed after probe was moved (□) had the same open times as pores that occurred when probe was always cis (▾, replotted from Fig. 4). For R18 (left), the first new pore after switching voltage polarity had shifted to longer times, but open times had not statistically lengthened until the second new pore formed.