TABLE 3.
Intracellular concentrations of nicotinamide adenine dinucleotides for the V5-pVT100-U and V5-pVT100-U-ADH2 transformants after 48 h of growth in YNB medium without amino acids containing 10% glucose
| Coenzyme | Mean nicotinamide adenine dinucleotide concn (μmol/g of cell dry wt) ± SD
|
Pa | |
|---|---|---|---|
| V5-pVT100-U | V5-pVT100-U-ADH2 | ||
| NAD+ | 2.7 ± 0.3 | 4.3 ± 0.3 | ≤0.05 |
| NADH | 0.29 ± 0.03 | 0.26 ± 0.04 | NS |
| NAD+/NADH | 9.24 | 16.73 | |
| NADP+ | 0.41 ± 0.07 | 0.09 ± 0.03 | ≤0.05 |
| NADPH | 0.8 ± 0.1 | 0.25 ± 0.04 | ≤0.05 |
| NADP+/NADPH | 0.51 | 0.37 | |
a
Variance analysis. NS, not significant.