FIG. 4.
Sag-dependent B-cell and DC activation is impaired in B10.BR mice. B10.BR (filled bars) and C3H/HeN (open bars) mice received subcutaneous injections of MMTV(LA) in their footpads, and after 4 days (A to D) or 6 days (D), the lymphocytes from their draining lymph nodes were analyzed by FACS for CD69 on CD4+ T cells (A), CD69 on CD11c+ DCs (B), the increase in the percentage of CD11c+ cells in the draining compared to the nondraining contralateral lymph node (C), and CD69 on B220+ B cells (D). A representative FACS plot of cells from the draining lymph nodes of B10.BR and C3H/HeN mice stained with anti-CD69 and -B220 is also shown. D, draining lymph node; ND, contralateral nondraining lymph node. The data presented are the averages of three mice and are representative of at least 10 independent experiments with MMTV(LA) or MMTV(FM). The error bars indicate standard deviations.