FIG. 8.
Translocation of PABP to the nucleus depends on the presence of NSP3 and not on the phosphorylation of eIF2α. Cells were transfected with the indicated siRNAs, and 48 hpt the cells were infected (+ virus) or mock infected (− virus) with RRV at an MOI of 3. Six hours postinfection the cells were fixed and processed for immunofluorescence, as described in Materials and Methods, using the indicated anti-PABP and anti-NSP2 antibodies (α-PABP and α-NSP2, respectively). Irr, irrelevant (control) siRNA.