TABLE 1.
Bacterial strains and plasmids
| Strain | Description | Source or reference |
|---|---|---|
| W4680A | K-12 ΔacrAB::kan | 21 |
| W4680AE | K-12 ΔacrAB::kan ΔacrEF::spe | Gift from H. Nikaido |
| W4680AD | K-12 ΔacrAB::kan ΔacrD::Tet | This study |
| ECM 2112 | MC4100 ΔacrAB::kan ΔtolC::Tn10 | Gift from O. Lomovskaya |
| pAhisB | pUC19 carrying His6-tagged acrA and acrB; Ampr | 37 |
| pMABO | pUC18 carrying mexA, mexB, and oprM; Ampr | 36 |
| pAMexB | pAhisB derivative carrying mexB | This study |
| pAMexBO | pAMexB derivative carrying oprM | This study |
| pAMexB D408A | pAMexB derivative carrying D408A mutation in mexB | This study |
| pMABO D408A | pMABO derivative carrying D408A mutation in mexB | This study |
| pAD111N-MexB | pAMexB carrying D111N mutation in acrA | This study |
| pAG240S-MexB | pAMexB carrying G240S mutation in acrA | This study |
| pAV244M-MexB | pAMexB carrying V244M mutation in acrA | This study |
| pAS249N-MexB | pAMexB carrying S249N mutation in acrA | This study |
| pA-MexBT329I | pAMexB carrying T329I mutation in mexB | This study |
| pA-MexBT489I | pAMexB carrying T489I mutation in mexB | This study |
| pA-MexBT557I | pAMexB carrying T557I mutation in mexB | This study |
| pA-MexBA802V | pAMexB carrying A802V mutation in mexB | This study |
| pAV244M-MexBT557I | pAMexB carrying mutations V244M in acrA and T557I in mexB | This study |
| pAV244M-MexBT489I | pAMexB carrying mutations V244M in acrA and T489I in mexB | This study |
| pA-MexBT329I/A802V | pAMexB carrying mutations T329I and A802V in mexB | This study |
| pAD111N-AcrB | pAD111N-MexB derivative carrying acrB | This study |
| pAG240S-AcrB | pAG240S-MexB derivative carrying acrB | This study |
| pAV244M-AcrB | pAV244M-MexB derivative carrying acrB | This study |
| pAS249N-AcrB | pAS249M-MexB derivative carrying acrB | This study |