FIG. 6.
Colons of supershedder mice contain intracellular (CD11c+ dendritic cells) and extracellular luminal Salmonella serovar Typhimurium. (a to f) Immunofluorescent photomicrographs of serial sections of colons from uninfected (a and d), moderate-shedder (b and e), and supershedder (c and f) mice. Host actin (blue) was stained with Phalloidin, and host leukocytes (red) were stained with CD11c (a, b, and c) and Gr-1 (d, e, and f) antibodies. Scale bars = 100 μm. (g and h) Immunofluorescent photomicrographs of supershedder colon stained for CD11c (red), Salmonella (green), and host nuclear DNA (blue). Scale bars = 100 μm (g) and 10 μm (h). (h) Magnified section of panel g, showing Salmonella serovar Typhimurium within a CD11c+ host cell. The XZ (above panel h) and YZ (to the right of panel h) images are sections from a three-dimensional reconstruction of the image in panel g. (i to k) Immunofluorescent photomicrographs of colonic contents from uninfected (i), moderate-shedder (j), and supershedder (k) mice stained for Gr-1 (red), Salmonella (green), and DNA (host cell or bacterial) (blue). Scale bars = 20 μm. The images are representative of the results of a microscopy analysis of tissues from five different mice.