FIG. 7.
Increased rate of tumor growth and increased HIF-α stabilization in SdhB shRNA cell-derived tumors. (A and B) Athymic nude mice were injected with 1 × 106 cells (143B wild type [WT], 143B mock shRNA, 143B SdhB shRNA clone 5E, or 143B SdhA shRNA polyclonal line 3 and clone 3C). Tumor growth was monitored for 35 days. Growth rates for SdhA shRNA polyclonal line 3 and 3C cell lines were virtually identical and have been combined in this graph. *, P < 0.05. (C) Upon excision, tumors were washed in cold PBS, and their mass was measured. *, P < 0.05. (D) Portions of the tumors were homogenized and lysed. Protein levels were measured by immunoblotting. Densitometric analysis of blots was carried out (Image J), corrected for β-actin levels, and normalized to WT levels. *, P < 0.05. Avg., average.