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. 1997 Mar 1;109(3):327–343. doi: 10.1085/jgp.109.3.327

Figure 2.

Figure 2

rSkM1-transfected CHO cells express large sodium currents that differ from small endogenous currents. (A) Current trace from a cell transfected with rSkM1 (CHOμ1). Currents were elicited by a 9-ms depolarizing pulse to potentials ranging from −60 to +100 mV in 10-mV intervals from the −100 mV holding potential. (B) Whole-cell sodium current-voltage relationship for transfected and nontransfected CHO-K1 cells. Points represent the average current ± SEM at a test potential. Filled circles: transfected CHOμ1 cells (n = 10). Open triangles: nontransfected CHO-K1 (n = 12). Curves are nontheoretical, point to point lines. (C) Conductance-voltage relationship. Data are the average peak conductances ± SEM at a membrane potential. Curves are fits of the data to single Boltzmann distributions. Filled circles: transfected CHOμ1 cells (n = 10). Open triangles: nontransfected CHO-K1 cells (n = 12). (D) Steady-state inactivation. Data are the average normalized current ± SEM measured during a 35-ms pulse to −10 mV following a 500-ms prepulse to the potentials graphed. Curves are fits of the data to single Boltzmann distributions. Filled circles: transfected CHOμ1 cells (n = 8). Open triangles: nontransfected CHO-K1 cells (n = 8).