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. 2008 Jan 14;180(1):83–90. doi: 10.1083/jcb.200706136

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Copyright © 2008, The Rockefeller University Press

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Figure 3. — Smc1β^−/− and Sycp3^−/−Smc1β^−/− oocytes show a high level of asynapsis at the pachytene stage. (A) Staining of wild-type (wt) and Smc1β^−/− pachytene oocytes (E18–18.5) with antibodies against Sycp1 (green), Rec8 (red), and CREST (white). Chromosomes labeled in yellow represent costaining of Sycp1 and Rec8, whereas chromosomes in Smc1β^−/− pachytene oocytes with slight yellow staining represent asynapsed chromosomes. (B) Scoring of asynapsed chromosomes in wt, Sycp3^−/−, Smc1β^−/−, and Sycp3^−/−Smc1β^−/− pachytene oocytes using FISH probes against chromosomes 12, 17, 19, and X. The percentage of asynaptic cells involving the four analyzed chromosomes (last column) was calculated as follows: the number of cells with asynapsis of one of the analyzed chromosomes was divided by the total number of cells analyzed for all four chromosomes, and the result was multiplied by 100. Bar, 10 μm.