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. 2005 Nov 21;202(10):1349–1361. doi: 10.1084/jem.20051357

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Copyright © 2005, The Rockefeller University Press

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Figure 3. — Interclonal avidity differences can be separated directly ex vivo using wild-type and CD8-null pMHCI tetramers in parallel. CD8⁺ T cells specific for EBV GL9 in PBMCs from donor T were stained with the corresponding wild-type (top) or CD8-null (bottom) pMHCI tetramers and sorted by flow cytometry. Constituent clonotypes within each sorted population are shown (right; total number of clones analyzed: wild-type, 65; CD8-null, 77). Common clonotypes were identical at the nucleotide level.