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. 2005 May 16;201(10):1523–1530. doi: 10.1084/jem.20041967

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Copyright © 2005, The Rockefeller University Press

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Figure 2. — Deficiency of Daf protein augments cellular immunity in vivo. (A) IFNγ ELISPOT assays were performed at rejection of male to female skin grafts (+/+, Daf1 ⁺ ^/ ⁺; −/−, Daf1 ⁻ ^/ ⁻) to assess reactivity to male peptides HYDby (left) and HYUty (right). Each symbol represents an individual animal (mean of triplicate wells, SD < 15%). –, means for all animals per group (n = 4–8); *, P < 0.05 versus Daf1 ^+/+ to Daf1 ^+/+. No response (<10 IFNγ producers/500,000 cells) was detected in any animal to control antigens OVA_323-339 and β-gal_96-103, and no responses were detected in naive mice (not depicted). (B) Spleen cell responses by IFNγ ELISPOT 12 d (top) or 30 d (bottom) after immunization of Daf1 ⁻ ^/ ⁻ or Daf1 ^+/+ mice with HYDby peptide plus CFA. No response was detected to control antigens (not depicted). Means plus SD for three to five animals/group are shown. The results are fully representative of two to four independent experiments. *, P < 0.05.