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. 2005 Mar 7;201(5):695–701. doi: 10.1084/jem.20042432

Figure 1.

Figure 1.

Defect in NKT cells in SAP-deficient mice. NKT cells were analyzed by flow cytometry in the liver, spleen, and thymus of wild-type (SAP+), SAP-deficient (SAP−), Fyn-deficient (Fyn−/−), and CD1d-deficient (CD1d−/−) mice. (A) Liver lymphocytes and splenocytes were stained with anti-CD19 and thymocytes with anti-HSA. After gating on CD19− or HSAlow cells, dot plots were constructed. Representative two-color dot plots show staining with anti-TCRβ versus anti-NK1.1 antibodies. The percentage of NKT cells corresponding to double positive cells in the circle gate is indicated in each plot. The data are representative of at least five mice per group. (B and C) Absolute numbers of NKT cells in the liver, spleen, and thymus mononuclear cells from SAP+ (black histogram), SAP− (white histogram), and Fyn−/− mice (gray histogram) stained for NK1.1 and TCRβ (B) or NK1.1 and Vβ8.2 TCR (C). Cells were counted in each organ and absolute numbers of NKT cells were determined based on their proportion (gated on double positive cells). Numbers are mean ± SD of three mice per group. (D) Same experiment as A. Representative two-color dot plots show the staining with anti-TCRβ antibodies versus αGalCer-loaded CD1d tetramers or unloaded CD1d tetramers (right). The percentage of NKT cells corresponding to double positive cells in the circle gate is indicated in each plot. The data are representative of at least five mice per group.