Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2005 Jan 17;201(2):279–289. doi: 10.1084/jem.20041502

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2005, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 3. — Phosphorylation of p38 MAPK and ATF-2 in Env-elicited syncytia. (A and B) Detection and subcellular localization of p38 MAPK phosphorylated on T180/Y182 (p38T180/Y182P) and ATF-2 phosporylated on T271 (ATF-2T271P). HeLa Env/CD4 cocultures (Syn., 36 h) or freshly mixed single cell (SC) suspensions were subjected to immunoblot detection of p38 (irrespective of its phosphorylation status), p38T180/Y182P (A), or ATF-2T271P (B). Moreover, immunofluorescence stainings for the detection of p38T180/Y182P (A) or ATF-2T271P (B) were performed and representative examples of prekaryogamic (preKG) and karyogamic (KG) syncytia are depicted. Note that p38T180/Y182P is cytoplasmic in prekaryogamic syncytia and nuclear in karyogamic syncytia. (C and D) Temporary relationship of p38T180/Y182P with regard to apoptosis. Double staining experiments revealed that nuclear p38T180/Y182P was detectable whereas cytochrome c was still retained in a punctate cytoplasmic (mitochondrial) localization (C). Kinetic analyses indicate that nuclear p38T180/Y182P accumulation correlates with p53S46P (D) yet occurred before cytochrome c release and nuclear chromatin condensation.