Figure 3.
Effect of theophylline effect on HDAC activity and dexamethasone sensitivity in AM. (A) Nuclear extracts obtained from AM of heavy smokers and patients with COPD were incubated with theophylline (10−5 and 10−6 M) for 20 min, and HDAC activity was measured. **P < 0.01, n = 3. (B) Effect of theophylline (Theo; 10−6 M) and dexamethasone (Dex; 10−6 M) alone and in combination on LPS-stimulated IL-8 release from AM obtained from COPD patients cultured overnight. #P < 0.05 versus LPS; ##P < 0.01 versus LPS; *P < 0.05 versus LPS plus dexamethasone (n = 6).