Table 1.
Antibody reactivity of cell-surface HAs by ELISA (% WT)
| HA | HC3 | HC31 | HC67 | HC68 | HC100 | HC263 | α-X31 |
|---|---|---|---|---|---|---|---|
| WT | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
| H171A | 100 | 85 | 99 | 82 | 105 | 81 | 104 |
| H171Y | 102 | 98 | 108 | 90 | 113 | 83 | 101 |
| K512A | 109 | 99 | 98 | 84 | 106 | 96 | 106 |
| K512E | 104 | 75 | 85 | 89 | 98 | 82 | 101 |
| Q1052A | 109 | 99 | 96 | 88 | 105 | 98 | 104 |
| Q1052E | 111 | 98 | 97 | 93 | 110 | 99 | 108 |
| H1062A | 106 | 113 | 102 | 100 | 107 | 93 | 105 |
| H1062F | 106 | 98 | 84 | 92 | 98 | 95 | 108 |
| H1062R | 99 | 78 | 99 | 85 | 106 | 83 | 112 |
| D1092A | 102 | 105 | 104 | 98 | 103 | 88 | 107 |
| T1112A | 110 | 125 | 104 | 105 | 117 | 99 | 105 |
| T1112H | 100 | 70 | 89 | 86 | 107 | 77 | 106 |
| T1112V | 99 | 78 | 81 | 88 | 101 | 88 | 109 |
| D1122A | 98 | 107 | 99 | 104 | 105 | 88 | 105 |
| H1062A, T1112A | 98 | 92 | 103 | 92 | 109 | 76 | 104 |
| H1062F, T1112V | 96 | 75 | 73 | 85 | 89 | 79 | 95 |
| H1062R, T1112H | 96 | 27 | 35 | 48 | 84 | 31 | 96 |
| D1092A, D1122A | 21 | 16 | 8 | 22 | 26 | 14 | 38 |
| H171A, H1062A, T1112A | 91 | 47 | 43 | 54 | 85 | 33 | 94 |
| H171Y, H1062A, T1112A | 100 | 98 | 104 | 87 | 107 | 81 | 107 |
| H171Y, H1062R, T1112H | 102 | 31 | 12 | 27 | 91 | 23 | 96 |
Values represent averages derived from a minimum of three separate experiments