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. 1998 Dec 21;188(12):2335–2342. doi: 10.1084/jem.188.12.2335

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Prior exposure to Drosophila DNA in vivo inhibits proliferation of CD8⁺ cells in vitro. CD8⁺ cells were purified from the LNs of the indicated mice 18 h after injection of either PBS or D. melanogaster DNA (200 μg/mouse for 2C mice, 500 μg/mouse for 129 or IFN-IR KO mice). (A) Purified CD8⁺ cells from 129 or IFN-IR KO mice were cultured for 2 or 3 d in 200 μl wells precoated with a mixture of anti-TCR and anti-CD28 mAbs. The data show means and SDs of duplicate cultures. (B) Purified 2C CD8⁺ cells (2.5 × 10⁴ cells/well) were cultured with Drosophila APCs (L^d/B7.1/ ICAM-1) and a titrated dose of QL9 peptide for the periods shown. Drosophila APCs were used at 2 × 10⁵ cells/well. The data show means and SDs of triplicate cultures; for many of the data points, SDs were too small to be illustrated.

Prior exposure to Drosophila DNA in vivo inhibits proliferation of CD8⁺ cells in vitro. CD8⁺ cells were purified from the LNs of the indicated mice 18 h after injection of either PBS or D. melanogaster DNA (200 μg/mouse for 2C mice, 500 μg/mouse for 129 or IFN-IR KO mice). (A) Purified CD8⁺ cells from 129 or IFN-IR KO mice were cultured for 2 or 3 d in 200 μl wells precoated with a mixture of anti-TCR and anti-CD28 mAbs. The data show means and SDs of duplicate cultures. (B) Purified 2C CD8⁺ cells (2.5 × 10⁴ cells/well) were cultured with Drosophila APCs (L^d/B7.1/ ICAM-1) and a titrated dose of QL9 peptide for the periods shown. Drosophila APCs were used at 2 × 10⁵ cells/well. The data show means and SDs of triplicate cultures; for many of the data points, SDs were too small to be illustrated.