Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1998 Dec 21;188(12):2369–2374. doi: 10.1084/jem.188.12.2369

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Targeting of the murine γ1 wild-type locus using a modified hMT vector with an inserted Ad splice donor site (Ad-5′ss). (A) Genomic organization of the murine IgG1 wild-type locus, the targeting vector, and the targeted Ad-hMT allele. The sγ1 probe used for Southern screening of homologous recombinant ES cell clones is represented as a black bar. Filled triangles indicate the primers used for screening of chimeric offspring. H, HindIII; E, EcoRI; Sc, ScaI; Amp, ampicillin resistance gene; TK, herpes simplex virus thymidine kinase gene; neo, neomycin resistance gene. (B) Detail of the γ1 wild-type and Ad-hMT targeted alleles. The genomic structure is compared with the hMT and s-hMT targeted loci (7). Sequences introduced by targeting vectors are shown in white: neo gene (neo), hMT promoter (hMT), artificial I_hMT exon (I_hMT); or in black: Ad splice donor site (Ad). The 114-bp fragment of wild-type sequence is represented as a hatched box. The phenotype of the mice is indicated with respect to transcription (transcription), splicing of the transcripts (processed transcripts), and class switch to IgG1 (class switch).

Targeting of the murine γ1 wild-type locus using a modified hMT vector with an inserted Ad splice donor site (Ad-5′ss). (A) Genomic organization of the murine IgG1 wild-type locus, the targeting vector, and the targeted Ad-hMT allele. The sγ1 probe used for Southern screening of homologous recombinant ES cell clones is represented as a black bar. Filled triangles indicate the primers used for screening of chimeric offspring. H, HindIII; E, EcoRI; Sc, ScaI; Amp, ampicillin resistance gene; TK, herpes simplex virus thymidine kinase gene; neo, neomycin resistance gene. (B) Detail of the γ1 wild-type and Ad-hMT targeted alleles. The genomic structure is compared with the hMT and s-hMT targeted loci (7). Sequences introduced by targeting vectors are shown in white: neo gene (neo), hMT promoter (hMT), artificial I_hMT exon (I_hMT); or in black: Ad splice donor site (Ad). The 114-bp fragment of wild-type sequence is represented as a hatched box. The phenotype of the mice is indicated with respect to transcription (transcription), splicing of the transcripts (processed transcripts), and class switch to IgG1 (class switch).