Figure 2.
TCR-mediated deletion of DP thymocytes in vivo and in vitro. (A) Total thymocytes from untreated mice were incubated for 24 h in the presence of medium alone (Control) or immobilized anti-CD3 (10 μg/ml) plus anti-CD28 (10 μg/ml) mAbs (In vitro anti-CD3/anti-CD28); total thymocytes were isolated from mice injected with anti-CD3 mAb (2C11, 50 μg/200 μl PBS) for 2 d (In vivo anti-CD3). The cells were stained with anti-CD4 and anti-CD8 mAbs and analyzed by flow cytometry. Profiles for CD4 and CD8 expression in gated live cells are presented. Numbers represent the percentage of each population (DP, CD4+, and CD8+). (B) Total thymocytes were incubated for 24 h in the presence of medium alone (Control) or Con A (2.5 μg/ml) (ConA). The cells were then examined as described in A.