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. 1998 Jun 15;187(12):1965–1975. doi: 10.1084/jem.187.12.1965

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Detection of PCLP mRNA by PCR. Fragments of the PCLP and HPRT sequences were amplified by PCR from serial dilutions of cDNA from purified HEC, tonsillar lymphocytes, and HUVEC. (-)RT, PCR reactions using samples from which the reverse transcriptase was omitted. Note that a faint PCLP band is barely visible in the lowest dilution of lymphocyte cDNA.