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. 1998 Apr 6;187(7):1103–1111. doi: 10.1084/jem.187.7.1103

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Productive and silent infection patterns associated with wild-type and Vpr-mutant cultures as assessed by in situ hybridization. Panels depict in situ patterns found in macrophage cultures infected on day 7 after isolation with high viral titers (50 ng) and monitored for RNA expression at 2 wk after infection, when a clear difference in virion production was evident as assessed by supernatant p24 levels (see Fig. 1). Uninfected culture (a), wild-type (b), ATG⁻ (c), R62P (d), and R80A (e).

Productive and silent infection patterns associated with wild-type and Vpr-mutant cultures as assessed by in situ hybridization. Panels depict in situ patterns found in macrophage cultures infected on day 7 after isolation with high viral titers (50 ng) and monitored for RNA expression at 2 wk after infection, when a clear difference in virion production was evident as assessed by supernatant p24 levels (see Fig. 1). Uninfected culture (a), wild-type (b), ATG⁻ (c), R62P (d), and R80A (e).