Abstract
Untoward reactions at the site of inoculation were not observed in monkeys vaccinated with influenza virus incorporated in a water-in-oil emulsion without acid-fast bacilli. Studies were then made to measure some of the dimensions of antigenicity of these emulsions to evaluate the extent of the immunologic adjuvant effect. This included measurements of height and persistence of the antibody response to inoculation and measurements of the extent to which the vaccine could be diluted and still induce antibody formation; i.e., antigenic extinction. In addition, comparisons were made of the rates of development of hemagglutination-inhibiting, virus-neutralizing, and complement-fixing antibody activities to determine the relationship among these three properties of the serum of immunized animals. It was found that levels of antibody many fold higher were induced by the virus-adjuvant mixtures as compared with virus in an aqueous menstruum, and that the level of antibody induced was related to the quantity of antigen incorporated in the emulsion. The stock vaccine when emulsified could be diluted 100,000-fold and was still active in antibody formation whereas a 100-fold dilution of the antigen without emulsification was essentially ineffective. Equivalent quantities of virus in 0.1 ml. or 1.0 ml. of emulsion induced antibody responses that were indistinguishable with respect to level or persistence. In comparing the course of antibody development it was found that hemagglutination-inhibiting, virus-neutralizing, and complement-fixing antibodies develop at different rates; careful analysis of the data derived from the present study together with other observations warrant the conclusion that these antibody activities are not present in constant proportion and are independent of one another. The implications of this observation and of the others mentioned above are discussed.
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Selected References
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- Friedewald W. F. ADJUVANTS IN IMMUNIZATION WITH INFLUENZA VIRUS VACCINES. J Exp Med. 1944 Dec 1;80(6):477–491. doi: 10.1084/jem.80.6.477. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hirst G. K., Rickard E. R., Whitman L., Horsfall F. L. ANTIBODY RESPONSE OF HUMAN BEINGS FOLLOWING VACCINATION WITH INFLUENZA VIRUSES. J Exp Med. 1942 May 1;75(5):495–511. doi: 10.1084/jem.75.5.495. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LIPTON M. M., FREUND J. The formation of complement fixing and neutralizing antibodies after the injection of inactivated rabies virus with adjuvants. J Immunol. 1950 Apr;64(4):297–303. [PubMed] [Google Scholar]
- Miller G. L., Stanley W. M. QUANTITATIVE ASPECTS OF THE RED BLOOD CELL AGGLUTINATION TEST FOR INFLUENZA VIRUS. J Exp Med. 1944 Feb 1;79(2):185–195. doi: 10.1084/jem.79.2.185. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SALK J. E., LAURENT A. M., BAILEY M. L. Direction of research on vaccination against influenza; new studies with immunologic adjuvants. Am J Public Health Nations Health. 1951 Jun;41(6):669–677. doi: 10.2105/ajph.41.6.669. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SALK J. E., LEWIS L. J., YOUNGNER J. S., BENNETT B. L. The use of adjuvants to facilitate studies on the immunologic classification of poliomyelitis viruses. Am J Hyg. 1951 Sep;54(2):157–173. doi: 10.1093/oxfordjournals.aje.a119474. [DOI] [PubMed] [Google Scholar]
- Salk J. E. A Plastic Plate for Use in Tests Involving Virus Hemagglutination and Other Similar Reactions. Science. 1948 Dec 31;108(2818):749–749. doi: 10.1126/science.108.2818.749. [DOI] [PubMed] [Google Scholar]
- Salk J. E. THE IMMUNIZING EFFECT OF CALCIUM PHOSPHATE ADSORBED INFLUENZA VIRUS. Science. 1945 Feb 2;101(2614):122–124. doi: 10.1126/science.101.2614.122. [DOI] [PubMed] [Google Scholar]
- WARD R., RADER D., LIPTON M. M., FREUND J. Formation of neutralizing antibody in monkeys injected with poliomyelitis virus and adjuvants. Proc Soc Exp Biol Med. 1950 Jul;74(3):536–539. doi: 10.3181/00379727-74-17964. [DOI] [PubMed] [Google Scholar]