Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2004 Jul 19;200(2):149–157. doi: 10.1084/jem.20040116

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2004, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 2. — GITR stimulation induces paradoxical responses in CD4⁺ CD25⁻ and CD8⁺CD25⁻ T cells in vitro and in vivo. (A) An anti-GITR agonist Ab (DTA-1; 10 μg/ml) was added to MLRs of purified CD4⁺CD25⁻ and CD8⁺CD25⁻ splenic T cells as effector cells (10⁵) with irradiated splenocytes as stimulators (2 × 10⁵). (Wild-type stimulators) C57BL/6 effectors and BALB/c stimulators. (GITR^+/− and GITR^−/− stimulators) BALB/c effectors and GITR^+/− or GITR^−/− stimulators (C57BL/6 × 129/SvJ). −Stim, without stimulators. +Stim, with stimulators. *, P < 0.001. (B) Proliferative profile of recovered CFSE-labeled CD4⁺CD25⁻ and CD8⁺CD25⁻ T cells from sublethally irradiated hosts treated with 10 μg/ml DTA-1 or rat IgG control. These data are representative of three independent experiments. (top left) Percentage of alloreactive fast-proliferating donor T cells. (center) Percentage of slow proliferating donor T cells. (top right) Nondividing donor T cells.