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Incubations of the fusion proteins MBP-H6-ABP and MBP-G6-ABP with different metal ions in the presence of ascorbate and hydrogen peroxide. Lanes 1–5 are with the His-containing linker (H6). In lane 1 no metal was added. In lanes 2–5 the metal concentration was 0.3, 1, 2, and 3.3 molar equivalents of metal to protein. Lanes 6–10 are with the Gly containing linker (G6) and the same concentrations of metal ions as for MBP-H6-ABP in lanes 1–5.
