Figure 5.
IL-12 production by LOD in response to systemic administration of STAg is restricted to CD8α+N418+ DC. LOD prepared from groups of STAg- or PBS-injected C57BL/6 mice were triple stained for IL-12 p40, N418, and CD8α or for DEC-205, N418, and CD8α. (A and B). IL-12+ cells are only seen in STAg-injected animals and are all bright for N418 (box). (C and D) Gating on N418+ cells demonstrates that IL-12+ cells seen in response to STAg (box) are part of the CD8α+ DC subset. All CD8α+ N418+ cells were also positive for NLDC-145, as reported (16, 17). Data are representative of three independent experiments. Other data from the same experiment are shown in Table 1.